Mansoura Veterinary Medical Journal

Ameliorative effects of Pergamum harmala seed extract on obese diabetic rats

Neveen Magdy, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Mohamed Salama, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Youssef Alsaedy, Department of Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Gehad El-Sayed, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

Document Type

Original Article

Subject Areas

Biochemistry and Physiology

Keywords

Obesity, Type 2 Diabetes, Methanolic extract of P. harmala seed

Abstract

Objective:To explore the potential antidiabetic activity of methanolic extract of Harmal seeds in obese-diabetic rats. Design: Randomized controlled experimental study. Animals: Forty male Sprague Dawley rats. Procedures: The P. harmala seeds methanolic extract was prepared and orally administered at two doses of 150 and 250 mg/kg to two groups of streptozotocin-induced diabetic rats. Two additional control groups were used as healthy control and obese-diabetic control groups. Animals were euthanized after 8 weeks of experimental period, blood and tissue samples were collected. Liver tissue samples were used to determine antioxidant and oxidative stress markers; while those from adipose tissue were used for estimation of PPAR gamma expression. Results:Supplementation of P. harmala methanolic extract with both doses (150 and 250 mg/kg) to diabetic rats (G3 and G4) significantly reversed the observed alterations in the levels of blood glucose, cholesterol, triglyceride, LDL, malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) (P <0.05). . In addition, the downregulation of PPAR gamma expression in diabetic rats (G2) was also restored in rats (G3 and G4) supplemented with P.harmala methanolic extract. Conclusion and clinical relevance: Our finding revealed that Harmal seed extract has a potent antidiabetic activity in streptozotocin-induced diabetic rats that can be used as a dietary supplement by diabetic patients.

How to Cite This Article

Magdy, Neveen; Salama, Mohamed; Alsaedy, Youssef; and El-Sayed, Gehad (2020) "Ameliorative effects of Pergamum harmala seed extract on obese diabetic rats," Mansoura Veterinary Medical Journal: Vol. 21: Iss. 3, Article 10.
DOI: https://doi.org/10.21608/mvmj.2020.21.319