Mansoura Veterinary Medical Journal

Molecular detection of virulence genes and Extended Spectrum Beta-lactam producing enzymes of Proteus mirabilis isolates from fishes in Federal Capital Territory (FCT) Abuja, Nigeria

Victor Bitrus Shammah, Department of General Studies, Federal College of Veterinary and Medical Laboratory, Technology, Vom, Plateau state, NigeriaFollow
Samuel Mailafia, Department of Veterinary Microbiology, Faculty of Veterinary Medicine University of Abuja Nigeria.
James A. Ameh, Department of Veterinary Microbiology, Faculty of Veterinary Medicine University of Abuja Nigeria.
Casmir Ifeanyichukwu Cejetan Ifeanyi Dr., Department veterinary Microbiology, faculty of Veterinary Medicine University of Abuja, Abuja Nigeria.Follow
Benshak John Audu, Biotechnology laboratory of National Veterinary Research Institute Vom, Plateau state, Nigeria
Hafsat Jagab, Biotechnology laboratory of National Veterinary Research Institute Vom, Plateau state, Nigeria
Job Gideon, Department of Biology Education, Faculty of Science Federal College of Education (Technical) Umaze, Imo state, Nigeria

Corresponding Author

Victor Bitrus Shammah

Document Type

Original Article

Keywords

ESBL, PCR, Proteus mirabilis, antibiotic susceptibility, fish, virulence genes.

Abstract

Objective: To study the molecular characterization of three virulence genes and phenotypic detection of extended spectrum beta-lactam-producing enzymes in Proteus mirabilis isolates from fish in the Federal Capital Territory (FCT).

Design: cross-sectional study.

Animal: A total of 400 fish were purchased from various markets and sites in the Federal Capital Territory. Thirteen (32.5%) P. mirabilis isolates were obtained. This study used amoxicillin with clavulanate and ceftazidime, a third-generation cephalosporin antibiotics, to treat all phenotypically typed isolates utilizing Extended Spectrum Beta-Lactam (ESBL). Polymerase Chain Reaction (PCR) was used for molecular characterization to identify the pathogenic genes of interest, including ureC, rsbA, and luxS.

Results: The prevalence of P. mirabilis by area councils shows that 4.81% for AMAC, 2.99% for Bwari and 2.5% for Gwagwalada. The isolates screened for the detection of ESBL enzymes showed that three (23%) were positive for ESBL. The ureC (0%) gene was not detected in any of the 13 isolates, rsbA (100%) and luxS (100%) genes were detected in all 13 isolates P. mirabilis obtained in our study.

Conclusion and clinical relevance: In conclusion, the present study showed that P. mirabilis occurs in this environment, which contains ESBL enzymes, and may create virulence factors that contribute to the pathogenicity of the organism. Public health awareness and education initiatives on the risks associated with P. mirabilis infection are therefore necessary, and should be intensified.

How to Cite This Article

Shammah, Victor Bitrus; Mailafia, Samuel; Ameh, James A.; Ifeanyi, Casmir Ifeanyichukwu Cejetan Dr.; Audu, Benshak John; Jagab, Hafsat; and Gideon, Job (2024) "Molecular detection of virulence genes and Extended Spectrum Beta-lactam producing enzymes of Proteus mirabilis isolates from fishes in Federal Capital Territory (FCT) Abuja, Nigeria," Mansoura Veterinary Medical Journal: Vol. 25: Iss. 4, Article 3.
DOI: https://doi.org/10.35943/2682-2512.1246