Mansoura Veterinary Medical Journal

Association between lactoferrin gene polymorphisms and mastitis susceptibility in Baladi goat

Osama Muhammad, Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, Egypt
Mohamed Fouda, Department of Husbandry, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, Egypt
Basma Hendam, Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, Egypt
Ahmed Ateya, Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Gomhoria St., Mansoura, P.O. box 35516, EgyptFollow

Document Type

Original Article

Keywords

LTF gene, Baladi goat, Gene polymorphism, Mastitis

Abstract

Objective: To explore the association between lactoferrin (LTF) gene polymorphisms and mastitis resistance and susceptibility in Baladi goats. Design: Crossover study Animals: Thirty female Baladi goats. Procedures: Blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. Polymerase chain reaction (PCR) was performed to amplify the 430-bp LTF gene. Results: PCR-DNA sequencing genetic assessment revealed nucleotide sequence variants in the LTF gene associated with mastitis susceptibility among the enrolled Baladi goats (GenBank accession numbers gb|PP037928| and gb|PP037929| for healthy and mastitic does, respectively). One non-synonymous single nucleotide polymorphism (SNP) G299A was discovered by DNA sequencing of LTF gene, which caused the amino acid C100Y to be substituted. Conclusion: LTF gene could be used as a candidate gene for mastitis resistance/susceptibility in goats, enabling marker-assisted selection in mastitis-resistant animals.

How to Cite This Article

Muhammad, Osama; Fouda, Mohamed; Hendam, Basma; and Ateya, Ahmed (2024) "Association between lactoferrin gene polymorphisms and mastitis susceptibility in Baladi goat," Mansoura Veterinary Medical Journal: Vol. 25: Iss. 2, Article 1.
DOI: https://doi.org/10.35943/2682-2512.1226