Mansoura Veterinary Medical Journal

Protective Effects of Nanoselenium on Japanese Quails Fed on Aflatoxin B1 Contaminated Diets on Performance Parameters, Hematological, Biochemical, Immunological and Pathological Studies

Hend D. Elbar, Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptFollow
Walaa F. Awadin, Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Abeer E. Aziza, Nutrition and Nutritional Deficiency Diseases, Faculty of Veterinary Medicine, Mansoura University
Ahmed El-Shaieb, Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Corresponding Author

Hend Diab Abd Elhady Ahmed Elbar

Authors ORCID

0000-0001-7762-0636

Document Type

Original Article

Keywords

AFB1, nano-Se, performance, immunohematological, biochemical and pathology, Japanese quails.

Abstract

Mycotoxins are considered to be a destructive problem in feed and food quality for animal and human health. Our study aimed to investigate the toxic effects of AFB1in addition to the protective effects of nano-Se against aflatoxicosis in quails. One hundred and twenty healthy Japanese quails were randomly divided into five groups of 24 birds each. First group; fed basal diet (negative control) without any addition, Second group; fed basal diet supplemented with 0.5 mg/kg diet nano-Se, Third group; fed basal diet containing 300 ppb AFB1 (positive control), Fourth group; fed basal diet containing 300 ppb AFB1 treated with 0.3 mg//kg diet nano-Se, Fifth group; fed basal diet containing 300 ppb AFB1 and treated with 0.5 mg//kg diet nano-Se. The experiment was conducted for six weeks. Compared to G1 and G2, the results revealed a decrease in FI, BWG, and FBW and an increase in FCR of quail performance. Significant decreases in Hb, PCV, RBCS, WBCS, DLC, total protein, albumin, and globulin were recorded in G3 compared to G1 and G2, and a high dose of nano-Se resulted in a significant increase in these parameters compared to G3 and G4. Significant increases in AST, ALT, creatinine, uric acid, TG, total cholesterol, LDL, and VLDL and a significant decrease in HDL were observed in G3 compared to G1 and G2. However, the opposite was observed for G5 compared with G3 and G4. In addition, AFB1 had a determintal effect on the expression of IL-1B and IL6 as which are indicators of inflammation and immunity. Severe degeneration and necrosis were observed in the liver, kidney, and lung with 300ppb AFB1. Slight to minimal pathological changes were observed in G3, whereas a marked improvement was achieved in G5. We concluded that high dose of nano-Se (0.5 mg/kg diet) had more protective effect than (0.3 mg/kg diet) nano-Se against toxic effects of AFB1 on growth performance, hemobiochemical and immunological parameters besides liver, lungs and kidneys histology of quails.

How to Cite This Article

Elbar, Hend D.; Awadin, Walaa F.; Aziza, Abeer E.; and El-Shaieb, Ahmed (2023) "Protective Effects of Nanoselenium on Japanese Quails Fed on Aflatoxin B1 Contaminated Diets on Performance Parameters, Hematological, Biochemical, Immunological and Pathological Studies," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 4, Article 7.
DOI: https://doi.org/10.35943/2682-2512.1213