Mansoura Veterinary Medical Journal

Histopathological study on the effect of Artemisinin multiple doses on the pathogenesis and immune reaction of Trichinella spiralis in albino rats.

Zeinab Dwidar, Pathology department, Faculty of Veterinary Medicine, Mansoura University, Egypt.
Hebatallah Mahgoub, Pathology department, Faculty of Veterinary Medicine, Mansoura University, Egypt.Follow
Iman Ibrahim, Pathology department, Faculty of Veterinary Medicine, Mansoura University, Egypt.
Ahmed El- Shaieb, Pathology department, Faculty of Veterinary Medicine, Mansoura University, Egypt.

Corresponding Author

Hebatallah Mahgoub

Authors ORCID

https://orcid.org/0000-0001-5832-9376

Document Type

Original Article

Subject Areas

Pathology

Keywords

Trichinella spiralis, Artemisinin, intestine, skeletal muscles, rats.

Abstract

Objective: This study aimed to determine the therapeutic effect of multiple doses of artemisinin (AR) on the pathogenesis and immune reaction of (T. spiralis ) during the intestinal and muscular phases in albino rats. Design: Randomized controlled study. Animal Models: The experiment was performed on 80 adult male and female albino rats weighing (160 ± 20 g) and their ages ranged from (4-6 to) weeks. . Procedures: rats were divided into four experimental groups (20 rats per group). 1-(Control negative) group. 2- T. spiralis infested (TR) group: Each animal was orally infested with 300 T. spiralis larvae. 3- T. spiralis treated (AR+TR) group: Each animal in this group was orally infested with 300 T. spiralis larvae, followed by treatment with AR at a dose of (400 mg/kg body weight) one day post infection (PI) for three successive days and three successive doses on days 21, 22, and 23 PI. 4-(AR) group, animals received only AR at the same dose. Results: The current study recorded that AR administration improved intestinal histopathological, morphohistopathological, and ultrastructural alterations during the intestinal phase. . In addition, it is effective in decreasing the larval count during the muscular phase. However, minimal improvements in the histopathological and ultrastructural alterations of the infected skeletal muscles were observed. The findings of muscular morphohistopathological, biochemical, and hematological analyses did not improve. Conclusion and clinical relevance: AR had significant anthelminthic efficacy against adult T. spiralis during the intestinal phase and minimal efficacy against encysted muscle larvae.

How to Cite This Article

Dwidar, Zeinab; Mahgoub, Hebatallah; Ibrahim, Iman; and El- Shaieb, Ahmed (2023) "Histopathological study on the effect of Artemisinin multiple doses on the pathogenesis and immune reaction of Trichinella spiralis in albino rats.," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 4, Article 5.
DOI: https://doi.org/10.35943/2682-2512.1223