Mansoura Veterinary Medical Journal

Development of Foot and Mouth Disease Vaccine Formulation in Egypt by Using Silver Nanoparticles as Immunogenic and Adjuvant

Rania Dweek, Animal Health Research Institute Mansoura provincial lab, Mansoura, EgyptFollow
M. Eisa, Department of Animal Medicine, faculty of Veterinary Medicine, zagazig University, Egypt
M. El-Beskawey, Department of Animal Medicine, faculty of Veterinary Medicine Matrouh University, Matrouh, 51744, Egypt.Follow
Sabry El- Khodery, Department of Internal medicine, Infectious Diseases and Fish Diseases., faculty of veterinary medicine, Mansoura University.
Abdelaziz A. Yassin, Department of Foot and Mouth Disease at Veterinary Serum and Vaccine Research Institute, Abbasia- Cairo.
Mohamed Farghli, Department of animal and poultry Hygiene, Environmental sanitation Faculty of veterinary medicine, Assuit University.
Hiam Fakhry, Department of Foot and Mouth Disease at Veterinary Serum and Vaccine Research Institute, Abbasia- Cairo
Samar Attwa, Department of Internal medicine, Infectious Diseases and Fish Diseases., faculty of veterinary medicine, Mansoura University

Corresponding Author

Rania Dweek

Document Type

Original Article

Subject Areas

Internal Medicine and Infectious Diseases

Keywords

FMD vaccine, Nanosilver (AgNPs), SNT, bovine IL-6.

Abstract

OBJECTIVE: To evaluate the effect of silver nanoparticles (AgNPs) combined with Foot and Mouth Disease (FMD)

inactivated oil adjuvant vaccine on the immunological response of cattle.

STUDY DESIGN: Randomized clinical trials.

PROCEDURES: AgNPs were prepared and added to the locally produced inactivated trivalent FMD vaccine used in

Egypt. Twenty-five cattle were classified into five groups (five/group). The first group was vaccinated with FMD

Trivalent inactivated oil adjuvant vaccine plus AgNPs (3 ml subcutaneously (S/C), the second group was vaccinated with

FMD trivalent inactivated vaccine (3 ml S/C). The third group was vaccinated with FMD trivalent inactivated vaccine plus AgNPs (2 ml S/C).The fourth group was injected with AgNPs only (1 ml S/C). The fifth (control) group was injected with normal saline (3 ml S/C).

RESULTS:Antibody titer by Serum neutralization test (SNT) for first group reached a protective value at 2 weeks (1.538 log10) and reached a peak at 2 months (2.77 log10). Bovine Interleukein -6 ( IL-6) revealed that animals vaccinated with AgNPs appeared more quickly than those vaccinated with FMD inactivated oil adjuvant vaccine.

CONCLUSION: AgNPs potentiated both cellular and humeral immunity against FMD.

How to Cite This Article

Dweek, Rania; Eisa, M.; El-Beskawey, M.; El- Khodery, Sabry; Yassin, Abdelaziz A.; Farghli, Mohamed; Fakhry, Hiam; and Attwa, Samar (2023) "Development of Foot and Mouth Disease Vaccine Formulation in Egypt by Using Silver Nanoparticles as Immunogenic and Adjuvant," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 4, Article 14.
DOI: https://doi.org/10.35943/2682-2512.1216