Mansoura Veterinary Medical Journal

In-Vitro Identification of Infectious Bursal Disease Virus Isolates from Dakahlia Governorate, Egypt through the Period from 2017 to 2018

Nancy A El-hefni, Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Sahar E. Abd-El Rahman, Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Ali A. El-Kenawy, Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Samah M. Mosad, Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptFollow

Corresponding Author

Samah M. Mosad

Document Type

Original Article

Keywords

AGPT, RT-PCR, VP2 gene, vvIBDV, phylogenic analysis

Abstract

Objective: Isolation and serological and molecular characterization of IBDV in field samples.

Design: Descriptive study.

Samples: A total of 55 chicken farms were studied between March 2017 and August 2018. Three diseased birds were collected from each farm, and their organs were pooled and considered one sample.

Procedures: The 55 collected samples were isolated from the chorioallantoic membrane (CAM) of embryonated chicken eggs (ECEs) for three passages and identified by changes in the embryo and CAM. Embryo and CAM homogenates from the 3rd passage of 50 samples showing embryonic changes were tested using the agar gel precipitation test (AGPT). Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to confirm IBDV in the 10 selected isolates. The PCR products of the selected samples were sequenced, and the nucleotide and deduced amino acid sequences were analyzed together with other sequences from GenBank.

Results: Of the 55 tested samples, 43, 48, and 50 showed embryonic changes in the 1st, 2nd and 3rd passages respectively. For AGPT, 48 isolates exhibited precipitation lines. All RT-PCR-tested isolates produced an expected band at 743 bp. Sequence analysis revealed that the studied isolate was closely related to Egyptian genogroup 3 vvIBDV with 95.8% to 98.1% identity. The studied isolate showed the characteristic vvIBDV serine-rich heptapeptide SWSASGS with the characteristic vvIBDV VP2 hypervariable region conserved amino acids.

Conclusion and clinical relevance:

The nucleotide and amino acid sequences of the IBDV-VP2 hyper variable region are valuable tools for characterizing vvIBDV.

How to Cite This Article

El-hefni, Nancy A; Rahman, Sahar E. Abd-El; El-Kenawy, Ali A.; and Mosad, Samah M. (2023) "In-Vitro Identification of Infectious Bursal Disease Virus Isolates from Dakahlia Governorate, Egypt through the Period from 2017 to 2018," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 3, Article 5.
DOI: https://doi.org/10.35943/2682-2512.1002