Mansoura Veterinary Medical Journal

Potential effect of Platelet Rich Fibrin and Chitosan on healing of surgically induced lacerated superficial digital flexor tendon in donkeys

Mahmoud Hussien, Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptFollow
Alaa Samy, Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Awad Rizk, Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Mohamed EL-Henawey, Department of Physics, faculty of science, Mansoura University, 35516 Mansoura, Egypt
Alshimaa M. Farag, Department of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Gamal Karrouf, Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Document Type

Original Article

Keywords

Platelet Rich Fibrin, superficial flexor tendon, Chitosan

Abstract

Objective: To investigate the effects of platelet-rich fibrin (PRF) and chitosan (Ch) on the healing of superficial digital flexor tendon (SDFT) defects in a donkey model.

Design: Randomized experimental study.

Animals: Eighteen clinically healthy male donkeys. A full-thickness defect of the SDFT was performed at the mid-metatarsus, and tenorrhaphy was performed, leaving a 1 cm defect. The animals were allocated into three groups (six animals per group). Group I (control group): no biomaterials were added; Group II (PRF group): the SDFT gap was filled with autogenous PRF; Group III (PRF/Ch group): a combination of PRF and chitosan was used to fill the SDFT gap. Ultrasonographic examinations were performed at 1, 2, and 3 months postoperatively, and the imaging characteristics were compared at each time point.

Results: The PRF and PRF/Ch groups showed significant (P>0.00) improvements in tendon echogenicity, fiber alignment, and thickness compared with the control group. In the PRF-and PRF/Ch-treated groups, SDFT showed a well oriented tendon fibers with normal thickness and normal crescent shape.

Conclusion and clinical relevance: Based on tendon echogenicity, fiber alignment, position, and thickness, PRF and chitosan can improve tendon healing and could provide a new bioscaffold-based strategy for SDFT regeneration in donkeys.

How to Cite This Article

Hussien, Mahmoud; Samy, Alaa; Rizk, Awad; EL-Henawey, Mohamed; Farag, Alshimaa M.; and Karrouf, Gamal (2023) "Potential effect of Platelet Rich Fibrin and Chitosan on healing of surgically induced lacerated superficial digital flexor tendon in donkeys," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 3, Article 4.
DOI: https://doi.org/10.35943/2682-2512.1001