Mansoura Veterinary Medical Journal

Naringenin attenuates hematobiochemical and histopathological alterations induced by lead intoxication in rats

Lubna A H Mansour, Clinical Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Gehad E. Elshopakey, Clinical Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Fatma M. Abdel Hamidieh, Clinical Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
Engy F M. Risha, Clinical Pathology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, EgyptFollow

Corresponding Author

Engy Fikry Risha

Authors ORCID

https://orcid.org/0000-0003-1653-1423

Document Type

Original Article

Keywords

Lead acetate, Naringenin, Hematological, Nephrotoxicity, Hyperlipidemia.

Abstract

Objective: The present study aimed to assess the protective effect of naringenin (NRG) against lead acetate toxicity in rats.

Design: Randomized controlled study.

Animals Forty adult male Wistar albino rats, weighing 140-160 g, were used in this study.

Procedure: Forty adult male rats were divided into four groups (10 rats/group). 1stgroup (A): Control group administered water and food pellets. 2ndgroup (B): Lead acetate dissolved in distilled water orally at a dose of 500 mg/kg/day. 3rdgroup (C) was administered naringenin at a dose of 50 mg/kg/day dissolved in 0.5% carboxymethyl cellulose (CMC). 4thgroup (D): Lead acetate plus naringenin was administered for four weeks. All rats were euthanized and renal and splenic tissues were collected. The influence of NRG against lead acetate toxicity on blood indices, nephrotoxicity, serum lipid profile, and histological changes were investigated.

Results: Lead acetate induced a significant decrease in hemogram results, whereas the total leukocyte and lymphocyte counts were significantly elevated. Meanwhile, a marked elevation in renal function biomarkers and serum lipid profiles was observed. However, high-density lipoprotein cholesterol (HDL-C) levels showed an opposite trend. Additionally, significant histopathological alterations were observed in the renal and splenic tissues of lead acetate (LA)-treated rats. Interestingly, we found that (NRG) markedly alleviated the lead-induced hematological, biochemical, and histopathological alterations.

Conclusion and clinical relevance: Naringenin administration successfully ameliorated the induced alterations in several health indicators in rats.

How to Cite This Article

Mansour, Lubna A H; Elshopakey, Gehad E.; Hamidieh, Fatma M. Abdel; and Risha, Engy F M. (2023) "Naringenin attenuates hematobiochemical and histopathological alterations induced by lead intoxication in rats," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 3, Article 2.
DOI: https://doi.org/10.35943/2682-2512.1011