Mansoura Veterinary Medical Journal

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

Basma Barghout, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt
Shaimaa El-Sayed, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt
El-Said El-Said, Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt

Document Type

Original Article

Subject Areas

Biochemistry and Physiology

Keywords

cadmium, green tea, testosterone, antioxidants, testes

Abstract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreasedserumtestosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promotedtesticular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-inducedtesticular damage in male rats.

How to Cite This Article

Barghout, Basma; El-Sayed, Shaimaa; and El-Said, El-Said (2020) "Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production," Mansoura Veterinary Medical Journal: Vol. 21: Iss. 1, Article 4.
DOI: https://doi.org/10.21608/mvmj.2020.21.105