Mansoura Veterinary Medical Journal

Effect of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

Huda Elbaz, Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura,35516, Egypt
Mohamed Hamed, Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Fatma Abdelhamid, Deparment of Clinical Pathology , Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
Osama Abdalla, Deparment of Clinical Pathology , Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

Document Type

Original Article

Subject Areas

Pathology

Keywords

Cefepime, E.coli ATCC 25922, Hepatic oxidative damage, immunological parameters, Rats

Abstract

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

How to Cite This Article

Elbaz, Huda; Hamed, Mohamed; Abdelhamid, Fatma; and Abdalla, Osama (2020) "Effect of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922," Mansoura Veterinary Medical Journal: Vol. 21: Iss. 1, Article 3.
DOI: https://doi.org/10.21608/mvmj.2020.21.116