Mansoura Veterinary Medical Journal

Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows

Mohamed El-Diasty, Animal Health Research Institute- Mansoura, Egypt.
Hager Talaat, Animal Health Research Institute- Mansoura, Egypt.
Samar Atwa, Department of Internal Medicine, Infections and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Elzahraa Elbaz
Mohamed Eissa, Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, P.O. 44519, Zagazig, Egypt.

Document Type

Original Article

Subject Areas

Internal Medicine and Infectious Diseases

Keywords

mastitis, Coagulase-negative Staphylococci, Antibiogram, cows

Abstract

Objective: To study the prevalence of Coagulase-negative Staphylococci in clinical and subclinical mastitis in dairy cows. Design: Case-control study. Animals: 415 lactating Holstein cows. Procedures: A total of 896 quarter milk samples were collected aseptically from 415 dairy cows for bacteriological examination (mastitic cows, n=50; healthy cows, n=174). Identification of different bacterial isolates followed by antibiotic sensitivity test for Coagulase-negative Staphylococci (CNS) was carried out, using disc-diffusion method against 13 different antibiotics . Results: 224 out of 415 cows (54%) were proven to be mastitic (12% clinical mastitis, 42 % subclinical mastitis). Coagulase-negative Staphylococci were the prevalent isolate (32%). CNS was recorded in 25.7 % of clinical cases and 34% of subclinical mastitis. The antibiogram of CNS isolates against 13 different antibiotics revealed high level of resistance to ampicillin (85%) and oxacillin (85%). However, they were less resistant to vancomycin (5%). The other antibiotics showed variable levels of resistance ranging from 40-75%. Conclusion and clinical relevance: Coagulase-negative Staphylococci were the most common bacteria isolated from mastitic cases. Multi-drug resistance was observed among CNS with high resistance to ampicillin and penicillin, so the use of such B-lactam antibiotics should be restricted specially if there were suspected cases of Coagulasenegative Staphylococci.

How to Cite This Article

El-Diasty, Mohamed; Talaat, Hager; Atwa, Samar; Elbaz, Elzahraa; and Eissa, Mohamed (2019) "Occurrence of Coagulase-negative Staphylococcal mastitis in dairy cows," Mansoura Veterinary Medical Journal: Vol. 20: Iss. 3, Article 2.
DOI: https://doi.org/10.21608/mvmj.2019.23.207