Mansoura Veterinary Medical Journal

DIFFERENTIATION OF NESTIN-POSITIVE MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS

Sahar Rashed, Department of Biochemistry, Faculty of Science, Mansoura University
Mahmoud Gabr
Abdel-Aziz Abdel-Aziz
Mahmoud Zakaria

Corresponding Author

Rashed, Sahar

Document Type

Original Article

Subject Areas

Bacteriology

Keywords

MSCs, diabetes, Rats

Abstract

Diabetes constitutes a worldwide epidemic affecting all ethnic groups. Stem cell therapy provides an effective way to regenerate insulin-producing cells lost during the disease. Accordingly, we isolated and compared the differentiation ability of nestin rich bone marrow mesenchymal stem cells (MSCs) into insulin producing cells (IPCs) with the nestin poor counterpart. The purity of the isolated populations was evaluated by immunofluorescence then, cells were differentiated into IPCs. At the end of differentiation, cells were evaluated by expression of pancreatic endocrine genes, immunofluorescence as well as the release of human insulin and c-peptide according to increasing glucose concentrations that showed a significant difference. Under confocal microscope the mean percentage of IPCs was 14.3% for nestin rich MSCs while nestin poor MSCs showed 2.17 %. In conclusion, nestin positive cells is a sub-population of MSCs, capable of generating IPCs in higher percentage than its negative counterpart. Key words: Nestin; Mesenchymal stem cells; differentiation; insulin producing cells.

How to Cite This Article

Rashed, Sahar; Gabr, Mahmoud; Abdel-Aziz, Abdel-Aziz; and Zakaria, Mahmoud (2018) "DIFFERENTIATION OF NESTIN-POSITIVE MESENCHYMAL STEM CELLS INTO INSULIN PRODUCING CELLS," Mansoura Veterinary Medical Journal: Vol. 19: Iss. 1, Article 8.
DOI: https://doi.org/10.21608/mvmj.2018.185427