Mansoura Veterinary Medical Journal

IMMUNOLOGICAL STUDIES ON E.COLI VACCINE IN CHICKEN

Amany Abd.ELEmoaty, Dep. of Bacteriology, Mycology and Immunology, Fac. Vet.Med, Mans. Univ.
Ahmad Ammar, Dep. of Microbiology, Fac. Vet. Med, Zagazig Univ.
AlaaEldin Moustafa, Dep. of Bacteriology, Mycology and Immunology, Fac. of Vet. Med. KafrElsheikh Univ.
Ibrahim Ibrahim, Vaccines Research Institute,Abbasia,Cario

Corresponding Author

Abd.ELEmoaty, Amany

Document Type

Original Article

Subject Areas

Bacteriology

Keywords

chicken, E.coli, vaccine

Abstract

The present study was carried out on One hundred and fifity , one day old chicks to evaluate the immunogenicity of multivalent pilus vaccine containing pili from the three common pathogenic serotypes of E. coli (O1, O2 and O78). Nienty vaccinated birds at 14 days & 28 days of age by 0.5ml s/c of pilus vaccine at base of neck and 60 unvaccinated were divided to 4 subgroups (30 vaccinated & 20 unvaccinated) were used for challenge two weeks after second dose . Each group was challenged each with one type of E.coli strains O1 , O2 and O78. Blood samples were collected during the experiment from the flocks one week before vaccination and weekly for 7th week after the first dose of vaccination for evaluation of immune response by using ELISA test. The prepared multivalent E.coil pilivaccine from combination of O1, O2 and O78E.coli strains were seemed to cover good range of protection and has been elicited a protective immune response against virulent E.coli challenge with homologous and heterologous strains. strong correlation was found between antibody response vaccinated groups and low lesions score that indicated a good protection.

How to Cite This Article

Abd.ELEmoaty, Amany; Ammar, Ahmad; Moustafa, AlaaEldin; and Ibrahim, Ibrahim (2017) "IMMUNOLOGICAL STUDIES ON E.COLI VACCINE IN CHICKEN," Mansoura Veterinary Medical Journal: Vol. 18: Iss. 1, Article 28.
DOI: https://doi.org/10.21608/mvmj.2017.127395