Mansoura Veterinary Medical Journal

Assessment of trichostrongyles resistance against anthelmintics commonly used in sheep reared in Dakahlia governorate, Egypt

Mahmoud R. Saleh, Dakahlia Directorate of Veterinary Medicine, Ministry of Agriculture, Mansoura, Egypt
Ibrahim Abbas, Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptFollow
Moustafa Al-Araby, Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Salah Abu-Elwafa, Parasitology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

Corresponding Author

Ibrahim Abbas

Authors ORCID

0000-0001-9479-6502

Document Type

Original Article

Keywords

Trichostrongyles; Haemonchus contortus; Anthelmintic resistance; Sheep; Egypt.

Abstract

Objective: To investigate, for the first time, the anthelmintic resistance of different trichostrongle-infecting sheep in the Dakahlia governorate, Egypt.

Design: Randomized controlled study.

Animals: 820 sheep were reared within 10 free-grazing herds with infrequent deworming practices.

Procedures: Fresh fecal samples were collected, and their floats were examined for trichostrongyle eggs, which were then identified using fecal cultures and/or ITS2-PCR amplification. Sheep (n=500) whose samples had egg counts ≥ 150 eggs per gram (EPG) of feces were selected and divided in each herd into five equal groups; each group received one of the tested anthelmintics, including albendazole (5 mg/kg BW), levamisole (7.5 mg/kg BW), ivermectin (0.2 mg/kg BW), and doramectin (0.2 mg/kg BW), whereas the fifth group was kept untreated. EPGs were detected for all groups at the time of treatment and weekly for 10 weeks post-treatment. The fecal egg count reduction percentage (FECR%) and egg reappearance period (ERP) were estimated.

Results: The examined herds were mostly infected with Haemonchus contortus; however, few Trichostrongylus species were detected in the fecal cultures of the two herds. In the untreated groups, the EPGs increased gradually throughout the experiment; however, no eggs were detected in the samples of all treated groups 7 days post treatment, resulting in 100% FECR. Macrocyclic lactones (ivermectin and doramectin) displayed longer ERPs than benzimidazoles (levamisole and albendazole).

Conclusion: No anthelmintic resistance was observed in the Trichostrongyles-infected sheep population from Dakahlia Governorate, Egypt, and the use of ivermectin to treat trichostrongyles-infected sheep is recommended.

How to Cite This Article

Saleh, Mahmoud R.; Abbas, Ibrahim; Al-Araby, Moustafa; and Abu-Elwafa, Salah (2023) "Assessment of trichostrongyles resistance against anthelmintics commonly used in sheep reared in Dakahlia governorate, Egypt," Mansoura Veterinary Medical Journal: Vol. 24: Iss. 3, Article 8.
DOI: https://doi.org/10.35943/2682-2512.1008